Peter's Tears
In their piece linking to a new 60-second Spider-Man 3 ad, SlashFilm asked "Why is Peter Parker crying?"
Just for them, spoilers follow.
He's in the hospital in this shot. Harry has been very seriously injured - by Peter, in the fight shown in the 7-minute NBC clip. It looks like Harry might not make it - but he does.
He only dies in the big pagger at the end of the film, saving Peter.
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